Pseudouridine detection improvement by derivatization with methyl vinyl sulfone and capillary HPLC-mass spectrometry.
Gert Emmerechts, Piet Herdewijn, Jef Rozenski
Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 825(2) , 233-8, (2005)
Full Text: HTML
Abstract
A method is presented for improved detection of pseudouridine in nucleoside mixtures based on the specific derivatization with methyl vinyl sulfone followed by analysis by capillary HPLC-mass spectrometry. Reaction conditions were optimized in order to obtain the best yield and specificity. The method was successfully applied to different nucleoside mixtures.
Related Compounds
Related Articles:
Usp5 links suppression of p53 and FAS levels in melanoma to the BRAF pathway.
2014-07-30
[Oncotarget 5(14) , 5559-69, (2014)]
Protein reactions with methyl and ethyl vinyl sulfones.
1988-02-01
[J. Protein Chem. 7(1) , 49-54, (1988)]
Trypanocidal activities of trileucine methyl vinyl sulfone proteasome inhibitors.
2005-01-01
[Parasitol. Res. 95(1) , 73-6, (2005)]
[Synthesis , 3495, (2006)]
Structural components in methyl vinyl sulfone modified cotton cellulose. Rowland SP, et al.
[Can. J. Chem. 44(9) , 1051-58, (1966)]