Simultaneous determination ofα-,β- andγ-asarone inAcorus tatarinowiiby microemulsion electrokinetic chromatography with [BMIM]PF6as oil phase

Ying Wang, Feng Li, Feng-Qing Yang, Hua-Li Zuo, Zhi-Ning Xia

Index: Talanta 101 , 510-5, (2012)

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Abstract

In the present study, a rapid and repeatable microemulsion electrokinetic chromatography (MEEKC) method was developed for the simultaneous determination of three isomers (α-, β- and γ-asarone) in Acorus tatarinowii by using ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF6) as oil phase. Experimental parameters including the microemulsion compositions (concentrations of surfactant, co-surfactant and oil phase), pH, concentration of borate buffer, capillary temperature and voltage were intensively investigated. Finally, the main compounds in the methanol extract of A. tatarinowii were well separated within 11min using a running buffer composed of 40mmol/L sodium dodecyl sulfonate (SDS), 2.0mol/L n-propanol, 8mmol/L [BMIM]PF6 in 10mmol/L borate buffer of pH 9.5. The developed method was applied to determine the contents of α-, β- and γ-asarone in A. tatarinowii from five different producing areas in China (Anhui, Hebei, Sichuan, Zhejiang and Chongqing). The results indicated that the contents of three asarones are quite different in the investigated A. tatarinowii samples. On the other hand, the MEEKC with ionic liquid as oil phase should be a promising method for the analysis of volatile components especially isomers in medicinal herbs.