Inhibitory effect of demoxepam on tryptophan binding to rat hepatic nuclei.

H Sidransky, E Verney, J W Cosgrove, A M Schwartz

Index: Biochem. Med. Metab. Biol. 47(3) , 270-3, (1992)

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Abstract

Since some patients with eosinophilia-myalgia syndrome ingested tryptophan along with benzodiazepines, we investigated whether demoxepam, the N-desalkylated compound of chlordiazepoxide, would influence the binding of tryptophan to hepatic nuclei. L-Tryptophan has been shown to bind (saturable, stereospecific, and of high affinity) to rat hepatic nuclei and nuclear envelopes. We report that demoxepam has an inhibitory effect on in vitro [3H]tryptophan binding to rat hepatic nuclei and has an apparent KD approximately 22 microM.