Determination of carbamazepine and its epoxide metabolite in plasma by high-speed liquid chromatography.

M Eichelbaum, L Bertilsson

Index: J. Chromatogr. A. 103(1) , 135-40, (1975)

Full Text: HTML

Abstract

A liquid-chromatographic method for the simultaneous determination of carbamazepine and its active metabolite (carbamazepine 10,11-epoxide) in plasma has been developed. The two compounds were identified in plasma by mass spectrometry. The lower limit of sensitivity is about 4 and 40 ng for the drug and its metabolite, respectively. 10,11-Dihydrocarbamazepine is used as internal standard for the determinations, which have precisions of 2.2 and 4.2%, respectively. No derivatization is needed. The specificity of the method for carbamazepine is shown by the significant correlation (r = 0.99) between the results obtained by this method and by mass fragmentography for the drug in plasma of patients.

Related Compounds

Related Articles:

Structure-activity relationship and site of binding of polyamine derivatives at the nicotinic acetylcholine receptor.

2000-01-01

[Eur. J. Biochem. 267(1) , 110-20, (2000)]

Liquid chromatographic methods for assay of carbamazepine, 10,11-dihydrocarbamazepine, and related compounds in carbamazepine drug substance and tablets.

1987-01-01

[J. Assoc. Off. Anal. Chem. 70(5) , 836-40, (1987)]

An orthorhombic polymorph of 10,11-dihydrocarbamazepine.

2006-05-01

[Acta Crystallogr. C 62(Pt 5) , o240-2, (2006)]

A predicted dimer-based polymorph of 10, 11-dihydrocarbamazepine (Form IV). Arlin J-B, et al.

[CrystEngComm 12(1) , 64-66, (2010)]

Determination of carbamazepine by chemiluminescence detection using chemically prepared tris(2,2'-bipyridine)ruthenium(III) as oxidant.

2003-06-01

[Anal. Sci. 19(6) , 903-6, (2003)]

More Articles...