[HPLC determination of diperodon enantiomers in blood serum by using teicoplanin chiral stationary phase].

K Hrobonová, J Lehotay, J Cizmárik

Index: Ceska. Slov. Farm. 53(5) , 225-7, (2004)

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Abstract

The chiral stationary phase on the base of teicoplanin and the polar-organic mobile phase methanol/acetonitrile/acetic acid/triethylamine 45/55/0.3/0.2 was used for the separation of diperodon enantiomers. The developed method was suitable to determine the enantiomers in blood serum up to 0.5 microg/ml. The degradation of diperodon enantiomers was studied in serum by an in vitro method and the experimental rate constants were determined.