Identification of glutamate transporters and receptors in mouse testis.

Jia-hua Hu, Na Yang, Ying-hua Ma, Jie Jiang, Jin-fu Zhang, Jian Fei, Li-he Guo

Index: Acta Pharmacol. Sin. 25(3) , 366-71, (2004)

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Abstract

To investigate the presence of glutamate transporters and receptors in mouse testis.Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transporters in mouse testis.Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 +/- 0.21) pmol/min per mg protein and Km value of glutamate uptake was (11.0+/-1.6) micromol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6 and KA2) were expressed in mouse testis.Glutamate transporters and receptors do exist in mouse testis.