The importance of alcohol dehydrogenase in regulation of ethanol metabolism in rat liver cells.
R A Page, K E Kitson, M J Hardman
Index: Biochem. J. 278 ( Pt 3) , 659-65, (1991)
Full Text: HTML
Abstract
We used titration with the inhibitors tetramethylene sulphoxide and isobutyramide to assess quantitatively the importance of alcohol dehydrogenase in regulation of ethanol oxidation in rat hepatocytes. In hepatocytes isolated from starved rats the apparent Flux Control Coefficient (calculated assuming a single-substrate irreversible reaction with non-competitive inhibition) of alcohol dehydrogenase is 0.3-0.5. Adjustment of this coefficient to allow for alcohol dehydrogenase being a two-substrate reversible enzyme increases the value by 1.3-1.4-fold. The final value of the Flux Control Coefficient of 0.5-0.7 indicates that alcohol dehydrogenase is a major rate-determining enzyme, but that other factors also have a regulatory role. In hepatocytes from fed rats the Flux Control Coefficient for alcohol dehydrogenase decreases with increasing acetaldehyde concentration. This suggests that, as acetaldehyde concentrations rise, control of the pathway shifts from alcohol dehydrogenase to other enzymes, particularly aldehyde dehydrogenase. There is not a single rate-determining step for the ethanol metabolism pathway and control is shared among several steps.
Related Compounds
Related Articles:
2016-01-01
[PLoS ONE 11 , e0145212, (2016)]
Induction of fetal hemoglobin expression by the histone deacetylase inhibitor apicidin.
2003-03-01
[Blood 101(5) , 2001-7, (2003)]
2000-08-01
[J. Mol. Endocrinol. 25(1) , 129-39, (2000)]
Thermosensitive molecular assemblies from poly(amidoamine) dendron-based lipids.
2011-07-04
[Angew. Chem. Int. Ed. Engl. 50(28) , 6332-6, (2011)]
2000-11-15
[Blood 96(10) , 3357-63, (2000)]