Covalent binding of nitrogen mustards to the cysteine-34 residue in human serum albumin.

Daan Noort, Albert G Hulst, Rob Jansen

Index: Arch. Toxicol. 76(2) , 83-8, (2002)

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Abstract

Covalent binding of various clinically important nitrogen mustards to the cysteine-34 residue of human serum albumin, in vitro and in vivo, is demonstrated. A rapid method for detection of these adducts is presented, based on liquid chromatography-tandem mass spectrometry analysis of the adducted tripeptide Cys*-Pro-Phe after digestion of the protein with Pronase.