Synthesis of fluorescent peptides and their phosphorylation by the catalytic subunit of cyclic AMP-dependent protein kinase.

…, R Kuroki, A Tada, K Fukumoto, J Sunamoto

Index: Kondo, Hiroki; Takaki, Katsunori; Kuroki, Ryoichi; Tada, Akihiro; Fukumoto, Kunihiro; Sunamoto, Junzo Bulletin of the Chemical Society of Japan, 1984 , vol. 57, # 10 p. 2957 - 2961

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Citation Number: 6

Abstract

The following two fluorescent peptides were prepared as substrates of the cyclic AMP- dependent protein kinase reaction: Leu–Arg–Arg–Ala–Ser–Leu–X; 1 (X= 2-(dansylamino) ethylamino or DAE) and 2 (X= 4-methyl-2-oxo-7-chromenylamino or MCA). Both peptides were phosphorylated by ATP and the catalytic subunit of cyclidAMP-dependent protein kinase from bovine heart in a stoichiometric manner. Phosphorylation of peptide 1 is ...

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