Vox Sanguinis 1985-01-01

Biochemistry of whole blood in poly(ethylene-co-ethylacrylate) experimental blood containers.

B Horowitz, A W Rowe, M H Stryker, J Van der Sande, A A Waldman, S C Wong, J D Gass, K R Woods

Index: Vox Sang. 48 , 143-149, (1985)

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Abstract

The biochemical status of whole blood stored in containers fabricated of ethylene ethylacrylate (EEA) film was monitored at several times during 4 weeks of storage at 4 degrees C. Fifteen biochemical indicators were studied to reflect on erythrocyte integrity, cellular metabolism, plasma protein stability, and microaggregate formation. Comparison to storage in polyvinyl chloride (PVC) containers was made by distributing aliquots from each unit of blood among the containers being compared. Whole blood in EEA developed significantly higher levels of plasma hemoglobin, erythrocyte osmotic fragility, and D-glycerate-2,3-diphosphate (2,3-DPG), and somewhat greater glucose utilization, lactate production, and pH. These biochemical differences were not of great magnitude and the data suggest that EEA containers are compatible with the storage of whole blood.


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