Biochemical and Biophysical Research Communications 1993-09-30

Interaction of pyrene-labeled monosialoganglioside GM1 micelles with cholera toxin.

W D Picking

Index: Biochem. Biophys. Res. Commun. 195(3) , 1153-8, (1993)

Full Text: HTML

Abstract

Monosialoganglioside GM1 labeled with 1-pyrene-dodecanoic acid was used to monitor interactions between cholera toxin and its receptor. Binding of cholera toxin to labeled ganglioside caused a decrease in pyrene fluorescence intensity at 480 nm concomitant with an increase in fluorescence intensity at 380 and 398 nm. The observed fluorescence changes are similar to those observed when fluorescent ganglioside is moved from an aqueous solvent to an organic solvent. The data are consistent with cholera toxin-bound ganglioside undergoing a process resembling solvent-dependent molecular dispersion.

Related Compounds

Related Articles:

Pyrene butanol--an efficient, selective and non-metabolized photosensitizing agent for human myeloid leukemia cells.

1992-01-01

[Leuk. Res. 16(5) , 453-62, (1992)]

Evidence that photodynamic stress kills Zellweger fibroblasts by a nonapoptotic mechanism.

1998-03-12

[Biochim. Biophys. Acta 1402(1) , 61-9, (1998)]

Analysis of cellular heterogeneity in the response of human leukemic cells to photosensitization induced by pyrene-containing fatty acid.

1989-01-01

[Leuk. Res. 13(12) , 1099-104, (1989)]

Newly identified Chinese hamster ovary cell mutants defective in peroxisome biogenesis represent two novel complementation groups in mammals.

1997-08-01

[Eur. J. Cell Biol. 73(4) , 352-9, (1997)]

Singlet oxygen scavenging by alpha-tocopherol and beta-carotene: kinetic studies in phospholipid membranes and ethanol solution.

1998-01-01

[Biofactors 7(1-2) , 31-40, (1998)]

More Articles...