Estimation of enantiomeric impurity in piperidin-3-amine by chiral HPLC with precolumn derivatization.

Chittabathina Venkata Raghunadha Babu, Nagi Reddy Vuyyuru, K Padmaja Reddy, M V Suryanarayana, K Mukkanti

Index: Chirality , doi:10.1002/chir.22352, (2014)

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Abstract

A simple, precise, accurate, robust chiral high-performance liquid chromatographic (chiral HPLC) method was developed for estimation of (S)-piperidin-3-amine (S-isomer) in (R)-piperidin-3-amine dihydrochloride (R-AMP). As AMP is a high-melting solid and nonchromophoric compound, development of a suitable chiral method is a challenging task. The proposed chiral HPLC-UV method involves a precolumn derivatization technique with para toluene sulphonyl chloride (PTSC) in the presence of a base to introduce chromophore into analytes. It utilizes chiralpak AD-H column with a simple mobile phase of 0.1% diethyl amine in ethanol with a 0.5 mL/min flow rate. Analytes were monitored by using a UV detector at 228 nm. The resolution between the two enantiomers was more than 4.0. The developed method was validated as per current International Conference on Harmonization (ICH) guidelines.© 2014 Wiley Periodicals, Inc.