Novel method for simultaneous aqueous in situ derivatization of THC and THC-COOH in human urine samples: validation and application to real samples.
S Chericoni, I Battistini, S Dugheri, M Pacenti, M Giusiani
Index: J. Anal. Toxicol. 35(4) , 193-8, (2011)
Full Text: HTML
Abstract
The present work describes the validation of a novel aqueous in situ derivatization procedure with trimethyloxonium tetrafluoroborate (TMO) as methylating agent for the simultaneous, quantitative analysis of Δ(9)-tetrahydrocannabinol (THC) and 11-nor-Δ(9)-tetrahydrocannabinol carboxylic acid (THC-COOH) in human urine. The derivatizing agent is directly added to the urine sample and the methyl-derivatives are then recovered by liquid-liquid extraction procedure. Gas chromatography-mass spectrometry was used to detect the derivatives in selected ion monitoring mode. The limits of detection were 0.7 ng/mL for THC and 0.5 ng/mL for THC-COOH, whereas the limits of quantification were 1.9 and 0.9 ng/mL, respectively. The method has been applied to 60 real samples both positive and negative to immunochemical screening test resulting to be very useful and reliable in routine analysis of THC-COOH in human urine for toxicological and forensic purposes.
Related Compounds
Related Articles:
Toward the total synthesis of spirastrellolide A. Part 2: Conquest of the northern hemisphere.
2006-08-18
[Angew. Chem. Int. Ed. Engl. 45 , 5510, (2006)]
1999-05-28
[J. Chromatogr. A. 843(1-2) , 237-45, (1999)]
Urinary organic acid screening by solid-phase microextraction of the methyl esters.
1998-08-25
[J. Chromatogr. B. Biomed. Sci. Appl. 713(2) , 427-32, (1998)]
2008-10-01
[Biomed. Chromatogr. 22(10) , 1155-63, (2008)]
Modification of carboxyl groups in sea anemone toxin RTX-III from Radianthus macrodactylus.
1990-01-01
[Toxicon 28(11) , 1255-63, (1990)]