Food and Chemical Toxicology 2009-10-01

Anti-obesity effect of sulfated glucosamine by AMPK signal pathway in 3T3-L1 adipocytes.

Chang-Suk Kong, Jung-Ae Kim, Se-Kwon Kim

Index: Food Chem. Toxicol. 47(10) , 2401-6, (2009)

Full Text: HTML

Abstract

In this study, we investigated the effect of sulfated glucosamine (SGlc) on adipogenesis of 3T3-L1 adipocytes during differentiation of preadipocytes into adipocytes by measuring lipid accumulation and adipogenesis related factors. Treatment with SGlc reduced the triglyceride content in Oil-Red O staining and enhanced glycerol secretion in adipocytes in a dose-dependent manner. In addition, SGlc induced the down-regulation of adipogenesis related factors and adipocyte specific gene promoters. Moreover, treatment of 3T3-L1 adipocytes with SGlc activated the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) alpha and beta along with their substrate, acetyl-CoA carboxylase (ACC). These results suggest that inhibitory effect of SGlc on adipocyte differentiation might be mediated through the up-regulation of AMPK pathway.

Related Compounds

Related Articles:

Human ficolin-2 recognition versatility extended: an update on the binding of ficolin-2 to sulfated/phosphated carbohydrates.

2014-12-20

[FEBS Lett. 588(24) , 4694-700, (2014)]

Characteristics of the glmS ribozyme suggest only structural roles for divalent metal ions.

2006-04-01

[RNA 12 , 607 - 619, (2006)]

The effects of glucosamine sulfate on intervertebral disc annulus fibrosus cells in vitro.

2015-06-01

[Spine J. 15 , 1339-46, (2015)]

Binding of heparin to antithrombin III: a chemical proof of the critical role played by a 3-sulfated 2-amino-2-deoxy-D-glucose residue.

1988-08-15

[Carbohydr. Res. 179 , 163-72, (1988)]

Common binding sites for beta-amyloid fibrils and fibroblast growth factor-2 in heparan sulfate from human cerebral cortex.

1999-10-22

[J. Biol. Chem. 274(43) , 30631-5, (1999)]

More Articles...