[Effects of citreoviridin on the expression of MCP-1 and ILs induced by TNF-alpha in vein endothelial cells].

Haifeng Hou, Qiang Jia, Ru Zhou, Cheng Li, Na Yuan, Mingfeng Yang, Guoyong Ding

Index: Wei Sheng Yan Jiu 41(2) , 195-8, 203, (2012)

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Abstract

To investigate the effects of citreoviridin (CIT) on the expression of MCP-1, IL-1beta, IL-6 and IL-8 induced by TNF-alpha in human umbilical vein endothelial cells (HUVECs).HUVECs isolated from the umbilical cord of neonates within 1 hour after birth (informed with consent form) were cultured in DMEM/ F12 media. After 80% of HUVECs were confluent, the cells were divided into four groups and treated with CIT (2 micromol/L) and/or TNF-alpha (10 microg/L). The levels of MCP-1, IL-1beta, IL-6 and IL-8 in the supernatant of cell culture media were measured by ELISA, the activation of NF-kappaB in HUVECs was detected by immunofluorescence staining, and the expression of MCP-1 mRNA was determinated by RT-PCR assay.The levels of MCP-1, IL-1beta, IL-6 and IL-8 in the supernatant and the expression of NF-kappaB P65 and MCP-1 mRNA of HUVECs were higher in TNF-alpha group and TNF-alpha + CIT group than those in the control group (P < 0.05), and those of TNF-alpha + CIT group was higher than TNF-alpha group (P < 0.05).The expression of NF-kappaB, MCP-1, IL-1beta, IL-6 and IL-8 in HUVECs up-regulated by TNF-alpha was promoted by CIT.