Purification and characterization of human brain serine racemase expressed in moderately halophilic bacteria.
Chizuru Nagayoshi, Matsujiro Ishibashi, Masao Tokunaga
Index: Protein Pept. Lett. 16(2) , 201-6, (2009)
Full Text: HTML
Abstract
We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.
Related Compounds
Related Articles:
1998-01-01
[Neuropharmacology 37 , 277-287, (1998)]
Effects of glutamate transporter inhibitors on the antitumor activity of doxorubicin.
2002-12-01
[Clin. Cancer Res. 8(12) , 3943-7, (2002)]
1996-08-15
[Eur. J. Biochem. 240(1) , 150-5, (1996)]
2001-07-01
[J. Membr. Biol. 182(1) , 17-30, (2001)]
1985-01-01
[J. Basic Microbiol. 25(2) , 111-8, (1985)]