Polyamines regulate cell growth and cellular methylglyoxal in high-glucose medium independently of intracellular glutathione.

Min-Kyu Kwak, Mun-Hyoung Lee, Seong-Jun Park, Sang-Min Shin, Rui Liu, Sa-Ouk Kang

Index: FEBS Lett. 590 , 739-49, (2016)

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Abstract

Polyamines can presumably inhibit protein glycation, when associated with the methylglyoxal inevitably produced during glycolysis. Herein, we hypothesized a nonenzymatic interaction between putrescine and methylglyoxal in putrescine-deficient or -overexpressing Dictyostelium cells in high-glucose medium, which can control methylglyoxal production. Putrescine was essentially required for growth rescue accompanying methylglyoxal detoxification when cells underwent growth defect and cell cycle G1-arrest when supplemented with high glucose. Furthermore, methylglyoxal regulation by putrescine seemed to be a parallel pathway independent of the changes in cellular glutathione content in high-glucose medium. Consequently, we suggest that Dictyostelium cells need polyamines for normal growth and cellular methylglyoxal regulation. © 2016 Federation of European Biochemical Societies.