Specific recognition of tyrosine-phosphorylated peptides by epitope imprinting of phenylphosphonic acid

Lin Xu, Yufeng Hu, Feng Shen, Qiushui Li, Xueqin Ren

Index: J. Chromatogr. A. 1293 , 85-91, (2013)

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Abstract

• We developed an epitope imprinting approach for pTyr peptide capture by MIPs. • PPA, a characteristic component of phenylphosphate group was used as template. • MIPs showed good selectivity and clear preference for pTyr peptide over pSer. • MIPs performed much more capacity (1.5-fold) to TiO2 for pTyr peptide recognition. • After three reuse cycles, there were still 94% of adsorption capacity retained.