Hydroxyl-functionalized DNA: an efficient orthogonal protecting strategy and duplex stability

D Zhang, L Xu, X Wei, Y Li, J He…

Index: Zhang, Di; Xu, Liang; Wei, Xia; Li, Yaming; He, Junlin; Liu, Keliang Nucleosides, Nucleotides and Nucleic Acids, 2009 , vol. 28, # 10 p. 924 - 942

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Citation Number: 5

Abstract

Tert-butyldiphenylsilyl (TBDPS) was found to be an effective orthogonal protecting strategy for the 5-substituted hydroxyl groups of de novo synthesized deoxyuridine analogues 1–3 and 7-(3-hydroxypropynyl)-of 8-aza-7-deazadeoxyadenosine 4 for their incorporation into oligodeoxynucleotides by phosphoramidite chemistry. It could be completely cleaved under normal and ultra-mild deprotection conditions applied to DNA synthesis, without extra ...