Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry.
Antonín Bednařík, Iva Tomalová, Viktor Kanický, Jan Preisler
Index: J. Chromatogr. A. 1364 , 271-5, (2014)
Full Text: HTML
Abstract
Here we present a novel coupling of thin-layer chromatography (TLC) to diode laser thermal vaporization inductively coupled plasma mass spectrometry (DLTV ICP MS). DLTV is a new technique of aerosol generation which uses a diode laser to induce pyrolysis of a substrate. In this case the cellulose stationary phase on aluminum-backed TLC sheets overprinted with black ink to absorb laser light. The experimental arrangement relies on economic instrumentation: an 808-nm 1.2-W continuous-wave infrared diode laser attached to a syringe pump serving as the movable stage. Using a glass tubular cell, the entire length of a TLC separation channel is scanned. The 8-cm long lanes were scanned in ∼35 s. The TLC - DLTV ICP MS coupling is demonstrated on the separation of four cobalamins (hydroxo-; adenosyl-; cyano-; and methylcobalamin) with limits of detection ∼2 pg and repeatability ∼15% for each individual species.Copyright © 2014 Elsevier B.V. All rights reserved.
Related Compounds
Related Articles:
2015-04-01
[Environ. Res. 138 , 67-73, (2015)]
2015-02-01
[PLoS Pathog. 11(2) , e1004645, (2015)]
2015-01-01
[Toxicol. Sci. 143(1) , 54-63, (2014)]
2015-04-21
[Analyst 140(8) , 2785-96, (2015)]
2015-01-01
[PLoS ONE 10(4) , e0122696, (2015)]