The two common polymorphic forms of human NRH-quinone oxidoreductase 2 (NQO2) have different biochemical properties

Clare F. Megarity, James R.E. Gill, M. Clare Caraher, Ian J. Stratford, Karen A. Nolan, David J. Timson

Index: FEBS Lett. 588(9) , 1666-72, (2014)

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Abstract

• SNP rs1143684 results in either a Phe or Leu at position 47 in human NQO2. • NQO2-L47 has a slightly lower kcat; it also has a lower Ki,app with resveratrol. • NQO2-L47 is more unstable to proteolysis and thermal denaturation. • NQO2-F47 (but not NQO2-L47) shows negative cooperativity towards resveratrol. • NQO2-L47 exists as multiple conformers in solution.