Microencapsulated mammalian cells for simultaneous production of VEGF165b and IFNα.

Fatemeh Afkhami, Yves Durocher, Satya Prakash

Index: Artif. Cells Blood Substit. Immobil. Biotechnol. 40 , 1-6, (2012)

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Abstract

Targeted and simultaneous delivery VEGF165b and IFN alpha in anti-angiogenic and other applications could offer several advantages. For this a system was design using artificial cell alginate-poly-L-lysine- alginate (APA) microcapsules. Result confirms the ability of this system for simultaneous production of these proteins for 28-days. The IFN alpha on a 3 days period increased from 8 ± 0.36 μg/ml at day 10 to 27 ± 2.4 μg/ml at day 16 and then dropped to 6.5 ± 0.5 μg/ml. The VEGF165b on a 3 days period increased from 2.7 ± 0.7 μg/ml at day 10 to 6.9 ± 1 μg/ml at day 16.