Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å.

Tereza Skálová, Jarmila Dušková, Jindřich Hašek, Andrea Stěpánková, Tomáš Koval, Lars Henrik Østergaard, Jan Dohnálek

Index: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 67(Pt 1) , 27-32, (2011)

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Abstract

The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 Å resolution, which is the highest resolution obtained to date.