Simultaneous determination of metformin and vildagliptin in human plasma by a HILIC-MS/MS method.

Roberto Pontarolo, Ana Carolina Gimenez, Thais Martins Guimarães de Francisco, Rômulo Pereira Ribeiro, Flávia Lada Degaut Pontes, João Cleverson Gasparetto

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 965 , 133-41, (2014)

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Abstract

The objective of this work was to develop and validate a HILIC-MS/MS method for the simultaneous determination of metformin and vildagliptin in human plasma. Chromatographic separation was achieved using an Atlantis HILIC Silica 150-mm × 2.1-mm, 3-μm particle size column maintained at 40°C. The isocratic mobile phase consisted of 20% water and 80% acetonitrile/water solution 95:5 (v/v), containing both 0.1% formic acid and 3mM ammonium formate. The flow rate was maintained at 400 μL min(-1). Data from validation studies demonstrated that the new method is highly selective, sensitive (limits of detection <1.5 ng mL(-1)) and free of matrix and residual effects. The new method was also precise (RSD<9.0%), accurate (RE<11.2%) and linear (r ≥ 0.99) over the ranges of 5-500 ng mL(-1) for each compound. The developed method was successfully applied to determine metformin and vildagliptin in plasma volunteers who orally received a single dose of metformin (850 mg), vildagliptin (50mg) or drug association (metformin 850 mg+vildagliptin 50mg). The new method can thus also be used as a tool for the clinical monitoring of metformin and vildagliptin.Copyright © 2014 Elsevier B.V. All rights reserved.