Validation of cyclooxygenase-2 as a direct anti-inflammatory target of 4-O-methylhonokiol in zymosan-induced animal models.
Hyung Sook Kim, Hwa Sun Ryu, Ji Sung Kim, Yong Guk Kim, Hong Kyung Lee, Jae Kyung Jung, Young Shin Kwak, Kiho Lee, Seung Yong Seo, Jieun Yun, Jong Soon Kang, Jin Tae Hong, Youngsoo Kim, Sang-Bae Han
Index: Arch. Pharm. Res. 38 , 813-25, (2015)
Full Text: HTML
Abstract
4-O-methylhonokiol (MH) is known to inhibit inflammation by partially understood mechanisms. Here, the anti-inflammatory mechanisms of MH were examined using enzymatic, cellular, and animal assays. In enzymatic assays, MH inhibited COX-2 activity with an IC50 of 0.062 μM, and also COX-1 with an IC50 of 2.4 μM. In cellular assays, MH was immunotoxic above 10 μM. At non-toxic concentrations (below 3 μM), MH strongly inhibited COX-2-mediated prostaglandin production with an IC50 of 0.1 μM, whereas did not or slightly affect other functions of B cells, T cells, dendritic cells, and macrophages. In an animal model, MH inhibited the increase in footpad thickness and popliteal lymph node weight in zymosan-injected mice. When analyzed the draining pLNs of zymosan-injected mice on day 5, MH inhibited the overall inflammatory responses. However, MH inhibited cyclooxygenase (COX)-2-mediated prostaglandin production without affecting tumor necrosis factor-α production in inflamed tissues within 6 h after zymosan injection. In summary, our data suggest that COX-2 may be a direct anti-inflammatory target of MH in vitro and in vivo.
Related Compounds
Related Articles:
2014-10-17
[Int. J. Food Microbiol. 189 , 98-105, (2014)]
2014-01-01
[PLoS ONE 9(6) , e99421, (2014)]
2014-04-01
[Pharmacogn. Mag. 10(Suppl 2) , S383-91, (2014)]
Glucose recognition proteins for glucose sensing at physiological concentrations and temperatures.
2014-07-18
[ACS Chem. Biol. 9(7) , 1595-602, (2014)]
Reservoirs of listeria species in three environmental ecosystems.
2014-09-01
[Appl. Environ. Microbiol. 80(18) , 5583-92, (2014)]