Amyloid load and translocator protein 18 kDa in APPswePS1-dE9 mice: a longitudinal study.

Sophie Sérrière, Clovis Tauber, Johnny Vercouillie, Céline Mothes, Christelle Pruckner, Denis Guilloteau, Michael Kassiou, Aurélie Doméné, Lucette Garreau, Guylène Page, Sylvie Chalon

Index: Neurobiol. Aging 36 , 1639-52, (2015)

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Abstract

We studied concomitantly the level of neuroinflammation and β-amyloid (Aβ) load in the APPswePS1dE9 transgenic mouse model of Alzheimer's disease using positron emission tomography. The translocator protein 18 kDa (TSPO) tracer [(18)F]DPA-714 was used to measure neuroinflammation and [(18)F]AV-45 for Aβ load in mice at 6, 9, 12, 15, and 19 months of age. At 19 months, we also analyzed the neuroinflammatory and neuroanatomic status of mice brains. The main affected brain areas were the cortex and hippocampus, with a concomitant progression of neuroinflammation with increased amyloid burden. At 19 months, no increase in TSPO binding was observed in the cerebellum; immunostaining revealed W0-2-positive plaques, indicating that the amyloid deposits seemed not stimulate inflammation. This finding was in agreement with the observed level of microglia and astrocytes staining. Our findings provide a better understanding of the relationships between neuroinflammation and plaque accumulation in the course of the disease in this mouse model. The monitoring of both processes should be of value to validate potential therapeutic approaches.Copyright © 2015 Elsevier Inc. All rights reserved.