Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup, O80.

Małgorzata Siwińska, Evgeniya A Levina, Olga G Ovchinnikova, Dominika Drzewiecka, Alexander S Shashkov, Antoni Różalski, Yuriy A Knirel

Index: Carbohydr. Res. 407 , 131-6, (2015)

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Abstract

Proteus penneri is an opportunistic pathogen, which may cause severe diseases, most frequently urinary tract infections in immunocompromised patients. P. penneri Br 114 exhibiting a good swarming growth ability as an S-form strain was isolated from a wound of a patient in Łódź, Poland. Serological studies using ELISA and Western blotting and chemical analyses along with (1)H and (13)C NMR spectroscopy showed that the O-antigen (O-polysaccharide) of this strain is unique among the known Proteus serotypes O1-O79. It possesses a linear pentasaccharide repeating unit containing a partially O-acetylated amide of D-glucuronic acid (GlcA) with L-serine having the following structure: [structure: see text]. These data are a basis for creating a new Proteus serogroup, O80, so far represented by the single Br 114 isolate. The O80 is the 21st O-serogroup containing P. penneri strains and the fourth serogroup based on Proteus spp. clinical isolates from Łódź, Poland.Copyright © 2015 Elsevier Ltd. All rights reserved.