Carbohydrate research

Optimization of lactulose synthesis from whey lactose by immobilized β-galactosidase and glucose isomerase

YS Song, HU Lee, C Park, SW Kim

Index: Song, Yoon-Seok; Lee, Hee-Uk; Park, Chulhwan; Kim, Seung-Wook Carbohydrate Research, 2013 , vol. 369, p. 1 - 5

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Citation Number: 10

Abstract

In the present study, commercially available whey was used as a lactose source, and immobilized β-galactosidase and glucose isomerase were used to synthesize lactulose from whey lactose in the absence of fructose. Optimal reaction conditions, such as lactose concentration, temperature, ionic strength of the buffer, and ratio of immobilized enzymes, were determined to improve lactulose synthesis using immobilized enzymes. Lactulose ...