Synthesis of oligodeoxynucleotides containing 6-N-([13 C] methyl) adenine and 2-N-([13 C] methyl) guanine

M Hofmann, M Acedo, P Fagan, D Wemmer…

Index: Hofmann, Mechtild; Acedo, Montse; Fagan, Patricia; Wemmer, David; Eritja, Ramon; Diaz, Antonio R. Journal of the Chemical Society - Perkin Transactions 1, 1997 , # 12 p. 1825 - 1828

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Abstract

Oligonucleotides containing 6-N-([13C] methyl) adenine and 2-N-([13C] methyl) guanine have been prepared for NMR studies using the deprotection step to introduce the [13C] methylamine group. For this purpose, the use of 2′-deoxy-6-O-(pentafluorophenyl) inosine 1 and 2′-deoxy-2-fluoro-6-O-[2-(4-nitrophenyl) ethyl] inosine 2 as precursors of the N- methylated nucleosides is described. Preliminary NMR characterization of the 13C- ...