Enzymatic Asymmetric Synthesis of Enantiomerically Pure Aliphatic, Aromatic and Arylaliphatic Amines with (R)??Selective Amine Transaminases

S Schätzle, F Steffen??Munsberg…

Index: Schaetzle, Sebastian; Steffen-Munsberg, Fabian; Thontowi, Ahmad; Hoehne, Matthias; Robins, Karen; Bornscheuer, Uwe T. Advanced Synthesis and Catalysis, 2011 , vol. 353, # 13 p. 2439 - 2445

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Citation Number: 60

Abstract

Abstract Seven (R)-selective amine transaminases (R-ATAs) recently discovered by an in silico-based approach in sequence databases were produced recombinantly in Escherichia coli and subjected to partial purification by ammonium sulfate precipitation. A range of additives and various buffers were investigated to identify best conditions to ensure good storage stability and stable activity during biocatalysis. All enzymes show pH optima ...