Interaction kinetics of liposome-incorporated unsaturated fatty acids with fatty acid-binding protein 3 by surface plasmon resonance

MC Tan, S Matsuoka, H Ano, H Ishida, M Hirose…

Index: Tan, Maria Carmen; Matsuoka, Shigeru; Ano, Hikaru; Ishida, Hanako; Hirose, Mika; Sato, Fuminori; Sugiyama, Shigeru; Murata, Michio Bioorganic and Medicinal Chemistry, 2014 , vol. 22, # 6 p. 1804 - 1808

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Citation Number: 7

Abstract

Abstract The role of heart-type fatty acid-binding protein (FABP3) in human physiology as an intracellular carrier of fatty acids (FAs) has been well-documented. In this study, we aimed to develop an analytical method to study real-time interaction kinetics between FABP3 immobilized on the sensor surface and unsaturated C18 FAs using surface plasmon resonance (SPR). To establish the conditions for SPR experiments, we used an FABP3- ...