Enzymically amplified voltammetric sensor for microliter sample volumes of salicylate
…, MJ Joseph, BW Allen, LA Coury Jr
Index: Moore, Thea J.; Joseph, Melissa J.; Allen, Barry W.; Coury Jr., Louis A. Analytical Chemistry, 1995 , vol. 67, # 11 p. 1896 - 1902
Full Text: HTML
Citation Number: 45
Abstract
A new voltammefric sensing strategy for salicylate employ-ing two enzymes and applicable to microliter sample volumes is demonstrated. The method involves the use of the enzyme salicylate hydrowlase to convert salicylate to catechol, which is oxidized at a carbon electrode. The product of this oxidation reaction, o-quinone, is then reduced by a second enzyme, glucose oxidase, to regenerate catechol. Reoxidation of catechol results in a ...
Related Articles:
Layer-by-Layer coated tyrosinase: An efficient and selective synthesis of catechols
[Guazzaroni, Melissa; Crestini, Claudia; Saladino, Raffaele Bioorganic and Medicinal Chemistry, 2012 , vol. 20, # 1 p. 157 - 166]
Synthesis of catechols from phenols via Pd-catalyzed silanol-directed C–H oxygenation
[Huang, Chunhui; Ghavtadze, Nugzar; Chattopadhyay, Buddhadeb; Gevorgyan, Vladimir Journal of the American Chemical Society, 2011 , vol. 133, # 44 p. 17630 - 17633]
[Jongerius, Anna L.; Jastrzebski, Robin; Bruijnincx, Pieter C.A.; Weckhuysen, Bert M. Journal of Catalysis, 2012 , vol. 285, # 1 p. 315 - 323]
Layer-by-Layer coated tyrosinase: An efficient and selective synthesis of catechols
[Guazzaroni, Melissa; Crestini, Claudia; Saladino, Raffaele Bioorganic and Medicinal Chemistry, 2012 , vol. 20, # 1 p. 157 - 166]
[Hwu, Jih Ru; Wong, Fung Fuh; Huang, Jiann-Jyh; Tsay, Shwu-Chen Journal of Organic Chemistry, 1997 , vol. 62, # 12 p. 4097 - 4104]