Layer-by-Layer coated tyrosinase: An efficient and selective synthesis of catechols

M Guazzaroni, C Crestini, R Saladino

Index: Guazzaroni, Melissa; Crestini, Claudia; Saladino, Raffaele Bioorganic and Medicinal Chemistry, 2012 , vol. 20, # 1 p. 157 - 166

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Citation Number: 23

Abstract

Agaricus bisporous tyrosinase was immobilized on commercial available epoxy-resin Eupergit® C250L and then coated by the Layer-by-Layer method (LbL). The two novel heterogeneous biocatalysts were characterized for their morphology, pH and storage stability, kinetic properties (Km, Vmax, Vmax/Km) and reusability. These biocatalysts were used for the efficient and selective synthesis of bioactive catechols under mild and ...