Screening for novel antimicrobials from encoded combinatorial libraries by using a two-dimensional agar format

…, NS Bhinderwala, Y Wang, KT Tsutsui…

Index: Silen, Joy L.; Lu, Amy T.; Solas, Dennis W.; Gore, Medini A.; Maclean, Derek; Shah, Nikil H.; Coffin, Jill M.; Bhinderwala, Naseema S.; Wang, Yongwen; Tsutsui, Ken T.; Look, Gary C.; Campbell, David A.; Hale, Ron L.; Navre, Marc; Deluca-Flaherty, Camille R. Antimicrobial Agents and Chemotherapy, 1998 , vol. 42, # 6 p. 1447 - 1453

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Citation Number: 69

Abstract

ABSTRACT A sensitive lawn-based format has been developed to screen bead-tethered combinatorial chemical libraries for antimicrobial activity. This method has been validated with beads linked to penicillin V via a photocleavable chemical linker in several analyses including a spike-and-recover experiment. The lawn-based screen sensitivity was modified to detect antibacterial compounds of modest potency, and a demonstration experiment ...