Fluorescent 7-diethylaminocoumarin pyrrolobenzodiazepine conjugates: Synthesis, DNA interaction, cytotoxicity and differential cellular localization

…, M Suggitt, M Coffils, MAH Baig, PW Howard…

Index: Wells, Geoffrey; Suggitt, Marie; Coffils, Marissa; Baig, Mirza A.H.; Howard, Philip W.; Loadman, Paul M.; Hartley, John A.; Jenkins, Terence C.; Thurston, David E. Bioorganic and Medicinal Chemistry Letters, 2008 , vol. 18, # 6 p. 2147 - 2151

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Citation Number: 19

Abstract

The pyrrolo [2, 1-c][1, 4] benzodiazepines (PBDs) are a class of DNA minor groove binding agents that react covalently with guanine bases, preferably at Pu-G-Pu sites. A series of three fluorescent PBD–coumarin conjugates with different linker architectures has been synthesized to probe correlations between DNA binding affinity, cellular localization and cytotoxicity. The results show that the linker structure plays a critical role for all three ...