Hydrolytic cleavage of pyroglutamyl-peptide bond. V. Selective removal of pyroglutamic acid from biologically active pyroglutamylpeptides in high concentrations of …

J Kobayashi, K Ohki, K Okimura, T Hashimoto…

Index: Kobayashi, Junko; Ohki, Kazuhiro; Okimura, Keiko; Hashimoto, Tadashi; Sakura, Naoki Chemical and Pharmaceutical Bulletin, 2006 , vol. 54, # 6 p. 827 - 831

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Citation Number: 4

Abstract

Application of aqueous methanesulfonic acid (MSA) for selective chemical removal of pyroglutamic acid (pGlu) residue from five biologically active pyroglutamyl-peptides (pGlu-X- peptides, X= amino acid residue at position 2) was examined. Gonadotropin releasing hormone (Gn-RH), dog neuromedin U-8 (d-NMU-8), physalaemin (PH), a bradykinin potentiating peptide (BPP-5a) and neurotensin (NT) as pGlu-X-peptides were incubated in ...