Characterization of the degradation products of luteinizing hormone releasing hormone

…, PF Hamburg, DA Graden, CJ Shaw…

Index: Motto; Hamburg; Graden; Shaw; Cotter Journal of Pharmaceutical Sciences, 1991 , vol. 80, # 5 p. 419 - 423

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Citation Number: 25

Abstract

Abstract The degradation products of luteinizing hormone releasing hormone [LH/RH; 1; gonadorelin releasing hormone (GnRH);< Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH 2] were determined in aqueous solution (pH 6.5) at 25, 37, 50, and 80 C. The predominant route of degradation involved the cleavage of the< Glu-His and Trp-Ser peptide bonds to give peptides 5–9 and hydrolysis of the terminal Gly-NH 2 to the free acid form in peptides ...