Nucleosides, Nucleotides and Nucleic Acids

An improved one-pot synthesis of nucleoside 5′-triphosphate analogues

I Gillerman, B Fischer

Index: Gillerman, Irina; Fischer, Bilha Nucleosides, Nucleotides and Nucleic Acids, 2010 , vol. 29, # 3 p. 245 - 256

Full Text: HTML

Citation Number: 30

Abstract

Nucleoside 5′-triphosphate (NTP) analogues are valuable tools for biochemical and medicinal research. Therefore, a facile and efficient synthesis of NTP analogues is required. Here, we report on an improved nucleoside 5′-triphosphorylation procedure to obtain pure products after liquid chromotagrpahy (LC) separation with no need for high performance liquid chromatography (HPLC) purification. To improve the selectivity of the reaction we ...

Related Articles:

The reaction of activated RNA species with aqueous fluoride ion: a convenient synthesis of nucleotide 5′-phosphorofluoridates and a note on the mechanism

[Tetrahedron Letters, , vol. 55, # 8 p. 1464 - 1466]

Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis

[Capyk, Jenna K.; Casabon, Israel; Gruninger, Robert; Strynadka, Natalie C.; Eltis, Lindsay D. Journal of Biological Chemistry, 2011 , vol. 286, # 47 p. 40717 - 40724]

Pyrophosphate formation via a phosphoramidate intermediate in polyammonium macrocycle: metal ion-catalyzed hydrolysis of ATP

[Journal of the American Chemical Society, , vol. 107, # 26 p. 8288 - 8289]

Enzymatic synthesis of new pyridine nucleosides. Clitidine and its amide derivative

[Bulletin of the Chemical Society of Japan, , vol. 54, # 1 p. 212 - 216]

Reactions of benzenediazonium ions with adenine and its derivatives

[Chin, Anton; Hung, Ming-Hong; Stock, Leon M. Journal of Organic Chemistry, 1981 , vol. 46, # 11 p. 2203 - 2207]

More Articles...