Hepatic metabolism of diclofenac: role of human CYP in the minor oxidative pathways

…, K Macé, A Boobis, MJ Gómez-Lechón, A Pfeifer…

Index: Bort, Roque; MacE, Katherine; Boobis, Alan; Gomez-Lechon, Maria-Jose.; Pfeifer, Andrea; Castell, Jose Biochemical Pharmacology, 1999 , vol. 58, # 5 p. 787 - 796

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Citation Number: 207

Abstract

The aim of this study was to re-examine the human hepatic metabolism of diclofenac, with special focus on the generation of minor hydroxylated metabolites implicated in the idiosyncratic hepatotoxicity of the drug. Different experimental approaches were used: human hepatocytes, human microsomes, and engineered cells expressing single human CYP (cytochromes P450). Human hepatocytes formed 3′-hydroxy-, 4′-hydroxy-, 5- ...