One-pot enzymatic production of β-D-galactopyranosyl-(1→ 3)-2-acetamido-2-deoxy-D-galactose (galacto-N-biose) from sucrose and 2-acetamido-2-deoxy-D- …

M Nishimoto, M Kitaoka

Index: Nishimoto, Mamoru; Kitaoka, Motomitsu Carbohydrate Research, 2009 , vol. 344, # 18 p. 2573 - 2576

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Citation Number: 43

Abstract

β-d-Galactopyranosyl-(1→ 3)-2-acetamido-2-deoxy-d-galactose (galacto-N-biose, GNB) is an important core structure in functional sugar chains such as T-antigen disaccharide and the core 1 sugar chain in mucin glycoproteins. We successfully developed a one-pot enzymatic production of GNB from sucrose and GalNAc by the concomitant action of four enzymes: sucrose phosphorylase, UDP-glucose-hexose 1-phosphate uridylyltransferase, ...