Regiospecific cleavage of RNA by RNase H from E. coli in the presence of a complementary oligonucleotide with inserted alternating 2'-O-methylcytidine residues
VG Metelev, NF Krynetskaya, AA Purmal…
Index: Metelev, Valerii G.; Krynetskaya, Natalya F.; Purmal, Andrei A.; Shabarova, Zoya A.; Tocik, Zdenek; et al. Collection of Czechoslovak Chemical Communications, 1990 , vol. 55, # 11 p. 2781 - 2786
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Abstract
Abstract Undecanucleotide dA-dC-dC-dA-Cm-Cm-dG-Cm-dG-Cm-dT (Cm= 2'-O- methylcytidine) was prepared and used as a probe for splitting of 5S rRNA fragment by RNase H (E. coli). It was found that substitution of 2'-deoxycytidine of d (ACCACCGCGCT) by 2'-O-methylcytidine in positions 5, 6, 8 and 10 provided regiospecific splitting of RNA fragment by RNase H in the presence of modified probe between U 25 and C 26.
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