A novel alkaline esterase from Sporosarcina sp. nov. strain eSP04 catalyzing the hydrolysis of a wide variety of aryl-carboxylic acid esters

M Takehara, K Kinoshita, M Miyamoto…

Index: Takehara, Munenori; Kinoshita, Kaori; Miyamoto, Masahiro; Hirohara, Hideo Bioscience, Biotechnology, and Biochemistry, 2012 , vol. 76, # 9 p. 1721 - 1727,7

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Citation Number: 4

Abstract

A novel esterase showing activity specific for esters of aryl-carboxylic acids was discovered in Sporosarcina sp. nov., which was identified by the 16S rDNA sequencing method in addition to morphological and physiological analyses. The aryl-carboxylesterase (named EstAC) was purified 780-fold from crude cell extracts by a 5-step procedure. EstAC was characterized as a monomeric protein with a molecular weight of 43,000, an optimum pH ...