Semisynthetic enzymes: characterization of isomeric flavopapains with widely different catalytic efficiencies

…, C Radziejewski, SR Oruganti, ET Kaiser

Index: Slama, James T.; Radziejewski, Czestaw; Oruganti, SubbaRao; Kaiser, E. T. Journal of the American Chemical Society, 1984 , vol. 106, # 22 p. 6778 - 6785

Full Text: HTML

Citation Number: 40

Abstract

Abstract: Flavopapain 6 has been prepared by alkylation of the active site cysteine-25 of papain with 8a-(bromoacetyl)-1 0-methylisoalloxazine. This semisynthetic enzyme was shown to serve as a catalyst for the oxidation of dihydronicotinamides, exhibiting saturation kinetics and up to 600-fold rate accelerations relative to a model flavin. This is contrasted to the behavior of flavopapain 9, the product of the modification of papain with 6a-( ...