Rapid screening of hydrolases for the enantioselective conversion of 'difficult-to-resolve'substrates
M Baumann, BH Hauer, UT Bornscheuer
Index: Baumann, Markus; Hauer, Bernhard H.; Bornscheuer, Uwe T. Tetrahedron Asymmetry, 2000 , vol. 11, # 23 p. 4781 - 4790
Full Text: HTML
Citation Number: 75
Abstract
Hydrolases showing high enantioselectivity towards three racemic alcohols (1-methoxy-2- propanol, 3-hydroxy-tetrahydrofuran, 3-butyn-2-ol) and pantolactone were identified by a step-wise screening procedure. Initially, those biocatalysts, which exhibited hydrolytic activity towards the corresponding acetates or butyrates, were selected out of> 100 enzymes. Here, rapid screening was performed in a pH-indicator, based format in ...
Related Articles:
[Everaere, Kathelyne; Carpentier, Jean-Francois; Mortreux, Andre; Bulliard, Michel Tetrahedron Asymmetry, 1999 , vol. 10, # 21 p. 4083 - 4086]
Generation of a solid Brønsted acid site in a chiral framework
[Ingleson, Michael J.; Barrio, Jorge Perez; Bacsa, John; Dickinson, Calum; Park, Hyunsoo; Rosseinsky, Matthew J. Chemical Communications, 2008 , # 11 p. 1287 - 1289]
[Baumann, Markus; Stuermer, Rainer; Bornscheuer, Uwe T. Angewandte Chemie - International Edition, 2001 , vol. 40, # 22 p. 4201 - 4204]
Synthesis of Oxochromium (VI) Alkoxides via Epoxide Cleavage. Structure, Reactivity, and Mechanism
[Troiani, Anna; Filippi, Antonello; Speranza, Maurizio Chemistry - A European Journal, 1997 , vol. 3, # 12 p. 2063 - 2070]
Synthesis of Oxochromium (VI) Alkoxides via Epoxide Cleavage. Structure, Reactivity, and Mechanism
[Troiani, Anna; Filippi, Antonello; Speranza, Maurizio Chemistry - A European Journal, 1997 , vol. 3, # 12 p. 2063 - 2070]