Regulatory Peptides 2012-01-10

Role of tachykinin and neurokinin receptors in the regulation of ovine omasal contractions.

Takenori Onaga, Taro Oh-ishi, Tomohiko Shimoda, Seira Nishimoto, Hideaki Hayashi

Index: Regul. Pept. 173(1-3) , 64-73, (2012)

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Abstract

The present study investigated a role of tachykinins (TK) and neurokinin (NK) receptors (NK-R) in the non-cholinergic regulation of omasal contractions in sheep. Semiquantitative reverse transcription (RT)-PCR revealed that both preprotachykinin (PPT)-A and PPT-B mRNA were distributed in the omasal muscle layers and that NK-R type-1 (NK-1R) and type-2 (NK-2R) mRNA were largely expressed in the same tissues. Cumulative application of substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) at 0.03-10μM induced tonic contractions of omasal muscle strips, and the contractile amplitude increased in order of NKB