Nucleosides, Nucleotides & Nucleic Acids 2003-01-01

Pyrrolidino-DNA.

Adrian Häberli, Alain Mayer, Christian J Leumann

Index: Nucleosides Nucleotides Nucleic Acids 22(5-8) , 1187-9, (2003)

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Abstract

We synthesized pyrrolidino-C-nucleosides, incorporated them into oligodeoxynucleotides and investigated their pairing properties. The thermal duplex and triplex stabilities were measured. While triplex formation is destabilized in the case of pyrrolidino-pseudo-U and -T, pyrrolidino-pseudo-iso-C leads to an increase of the Tm value for third strand dissociation. Duplexes are destabilized with all pyrrolidino-C-nucleosides.