Bioscience Reports 1986-03-01

Incorporation of 3-deoxy-3-fluoro-D-glucose into glycogen and trehalose in fat body and flight muscle in Locusta migratoria.

M Agbanyo, N F Taylor

Index: Biosci. Rep. 6(3) , 309-16, (1986)

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Abstract

Flight muscle and fat body extracts from Locusta migratoria were incubated with D-[U-14C]-glucose or D-[3-3H]-3-deoxy-3-fluoroglucose and the products were analyzed. In the case of the latter compound, radio-chromatographic analysis yielded glycogen and trehalose fractions that were shown by 19F nuclear magnetic resonance to contain fluorine. Acid hydrolysis of these fractions liberated tritium labelled 3-deoxy-3-fluoro-D-glucose. In addition to the formation of "fluoroglycogen" and "fluorotrehalose" in these tissue extracts, there was an accumulation of tritium labelled fructose.