Journal of Virological Methods 1992-07-01

Psoralen preparation of antigenically intact noninfectious rotavirus particles.

W S Groene, R D Shaw, William S. Groene, Robert D. Shaw

Index: J. Virol. Methods 38(1) , 93-102, (1992)

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Abstract

The use of the synthetic psoralen 4'-aminomethyl-4,5',8-trimethylpsoralen hydrochloride (AMT) is described for the inactivation of infectious rotavirus, a member of the viral family Reoviradae with a double-stranded RNA genome. This method not only provides complete inactivation of the virus but leaves antigenically intact particles. The lack of viral replication following inactivation was determined with an immunohistochemical focus assay. The antigenic authenticity of the particles was determined by monoclonal antibody ELISA and a viral hemagglutination assay.