International Journal of Systematic and Evolutionary Microbiology 2013-02-01

Micromonospora maritima sp. nov., isolated from mangrove soil.

Apakorn Songsumanus, Somboon Tanasupawat, Yasuhiro Igarashi, Takuji Kudo

Index: Int. J. Syst. Evol. Microbiol. , (2012)

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Abstract

Strain D10-9-5(T) was isolated from mangrove soil in Samut Sakhon province, Thailand. A polyphasic approach was used to determine the taxonomic position of the strain. The strain presented single rough spores on substrate mycelium and no aerial mycelium. Chemotaxonomic data supported the assignment of strain D10-9-5(T) to the genus Micromonospora based on the presence of meso-diaminopimelic acid and glycolyl muramic acid in the peptidoglycan, ribose, mannose, galactose, xylose and glucose as whole-cell sugars, MK-10(H(4)) (14.8 %), MK-10(H(6)) (46.7 %) and MK-10(H(8)) (27.5 %) as the predominant isoprenoid quinones, iso-C(15 : 0) (17.9 %), anteiso-C(17 : 0) (14.6 %), iso-C(17 : 0) (9.6 %), C(17 : 0) (8.0 %), iso-C(16 : 0) (7.7 %) and C(17 : 1)ω8c (7.0 %) as the major cellular fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and phosphatidylethanolamine as the predominant phospholipids in the cell wall. The 16S rRNA gene sequence and phylogenetic analysis showed that strain D10-9-5 was closely related to Micromonospora marina JCM 12870(T) (99.6 %), Micromonospora coxensis JCM 13248 (T) (99.4 %), Micromonospora aurantiaca JCM 10878(T) (99.3 %), Micromonospora humi JCM15292(T) (99.3 %), Micromonospora halophytica JCM 3125(T) (99.1%) and Micromonospora chalcea JCM 3031(T) (99.1 %). Strain D10-9-5(T) could be clearly distinguished from related members of the genus Micromonospora by its physiological and biochemical characteristics as well as its phylogenetic position and level of DNA-DNA relatedness. Therefore, the strain represents a novel species for which the name Micromonospora maritima sp. nov. is proposed; the type strain is D10-9-5(T) ( = JCM 17013(T) = NBRC 108767(T) = PCU 322(T) = TISTR 2000(T)).