Molecular BioSystems 2010-04-01

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.

Ying Huang, William K Russell, Wei Wan, Pei-Jing Pai, David H Russell, Wenshe Liu

Index: Mol. Biosyst. 6(4) , 683-6, (2010)

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Abstract

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.